VWF Collagen Binding
| Profile or Test Panel |
Bleeding Diathesis with a Normal aPTT/PT Profile
VWD Profile
If the aPTT is prolonged and the factor VIII is normal, this panel reflexes to an aPTT Reflexive Evaluation Profile.
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| Indications | Requires VW Activity by Ristocetin Cofactor, VW Antigen and FVIII levels to interpret results |
| Method | ELISA |
| Test Information | VWF collagen binding performed to see if patient had a VWF functional variant affecting collagen binding. VWF collagen binding activity usually parallels Ristocetin cofactor activity, however there are uncommon variants with isolated defects in collagen binding. Note: VWF collagen binding results should be considered for research use only. |
| Additional Test Information | Normal ranges are included on testing report and can be made available upon request. |
| Sample Requirements | Whole Blood: 3.2% sodium citrated (blue top) OR frozen plasma. |
| Requested Volume | Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood OR frozen plasma in 4 X 1mL aliquots in plastic tubes. |
| Sample Information | Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE WITHIN 2 HOURS of collection, then send frozen plasma. To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes. Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze. |
| Shipping Information | Whole Blood: Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.
Frozen Plasma: Send on dry ice Send samples to: For additional information, please see Ordering Information |
| Requisition Form | RFT- Hemostasis Reference Laboratory |
| Transaction Code | 3210-26 |
| CPT Codes | 82523 |
| Test Schedule | Run Monthly |
| Turn Around Time (analytic time) | Contact Laboratory |
| Report/Results | Fax number required to receive report |
