HLA/Immunogenetics Laboratory Profiles

Final Crossmatch for Deceased Donor Transplant

Kidney, Pancreas, or Kidney with additional organ

    Tests ordered:

• Lymphocyte Crossmatch-STAT

Cardiothoracic

    Tests ordered:

• Lymphocyte Crossmatch

Liver

    Tests Ordered:

• HLA Class I + II (A,B,C,DR,DQ) Typing
• Lymphocyte crossmatch
  • (HLA Antibody Specificity-Monitoring (DSA Donor-Specific Antibody) may be added if crossmatch is positive)

    For Liver Transplant, these tests are usually performed  after transplant; pediatric transplants may have center specific protocols

Testing Details for Final Crossmatch workup

• Specimen Requirements:
  • Three 10cc ACD blood tubes (yellow top, solution A or B) and one 10cc clotted blood tube (red top)
• Labeling Requirements:
  • Specimen must have the patient’s first and last name, a collection date and a unique identifier written on the tube.
• Analytic Time:
  • For Kidney, Pancreas, or Kidney with additional organ: 4 to 8 hours
  • For Cardiothoracic: within 24 hours of transplant
  • For Liver: Retrospective testing performed within 7 business days after transplant is confirmed in UNet.

All specimens must be accompanied with  a Request for Testing Form