Prothrombin Time 1:1 Mix

Method Clottable
Test Information The prothrombin time is performed using a 1:1 mix of patient plasma with normal plasma. Lack of prothrombin time correction with the  1:1 mix indicates the presence of a coagulation inhibitor.  If the prothrombin time is abnormal and etiology unclear, the testing will reflex to this test.
Additional Test Information Normal ranges are included on testing report and can be made available upon request.
Sample Requirements Whole Blood:  3.2% sodium citrated (blue top)  OR
frozen plasma.
Requested Volume Two 5 ml 3.2% sodium citrated (blue top) tubes as whole blood  OR
frozen plasma in 4 X 1mL aliquots in plastic tubes.
Sample Information Whole blood is preferred for testing, but if it is not possible to send whole blood tubes TO ARRIVE HERE within 2 hours of collection, then send frozen plasma.  To prepare plasma, centrifuge blue top tubes at 1500g for 15 minutes and remove the plasma and centrifuge a second time at 1500g for an additional 15 minutes.  Aliquot approximately 1 ml of plasma into 4 plastic tubes, and freeze.
Shipping Information Whole Blood:  Send at ambient temperature (18-25°C) and must be received within the lab in less than or equal to 2 hours after collection.

Frozen Plasma:  Send on dry ice

Send samples to:
Bloodworks Northwest
Attn: Hemostasis Laboratory
921 Terry Ave
Seattle, WA 98104

For additional information, please see Ordering Information.

Requisition Form RFT- Hemostasis Reference Laboratory
Transaction Code 3200-04
CPT Codes 85611
Test Schedule Monday-Friday
Turn around Time (analytic time) 3 Business Days
Report/Results Fax number required to receive report

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