Indications Recovery of antibody bound to red blood cells of patients found to have a positive direct antiglobulin test. The eluate is prepared from patient’s red blood cells that have been coated in vivo to determine the specificity of the antibody.
Method Standard test tube methodology; Acid elution
Test Information Test performed on sample with a Positive Direct Antiglobulin (DAT) result.
Sample Requirements EDTA tube. Serum separator gel tube is not acceptable.
Requested Volume 1 full 7 ml EDTA sample
Minimum Volume or Pediatric volume Minimum volume: 1 full 7 ml EDTA sample
Pediatric: 1 full 3 ml EDTA sample
Shipping Information Ship at ambient temperature

Send samples to:
Bloodworks Northwest
Immunohematology Reference Laboratory
921 Terry Avenue
Seattle, WA 98104

Requisition Form Request for Testing-Immunohematology Reference Laboratory
Immunohematology Consultation Request
Transaction Code 3129-00
CPT Codes 86860
Test Schedule Monday through Sunday
Turn around Time (analytic time) 24 hours if weekday; 48 hours if weekend
Report/ Results Fax number required to receive report

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