Direct Antiglobulin Test-Immunohematology Reference Lab
| Indications | Detects the presence of red blood cell-bound IgG and/or complement (C3) by agglutination using both polyclonal and monospecific antiglobulin (Coombs) reagents. Positive results can occur in patients with autoimmune hemolytic anemia, drug-induced immune hemolysis (e.g., alpha-methyldopa, procainamide), hemolytic transfusion reactions, or hemolytic disease of the newborn. Positive results can also occur due to non-specific IgG absorption (e.g., hypergamma-globulinemia), and occasionally in otherwise healthy individuals. Elution testing is usually indicated after a positive result with IgG is detected. |
| Method | Standard test tube methodology, Hemagglutination |
| Test Information | Extended evaluation may include elution and absorption studies. |
| Sample Requirements | EDTA tube, separator gel tube is not acceptable. |
| Requested Volume | 1 full 7 ml EDTA sample |
| Minimum Volume or Pediatric volume | Minimum volume: 1 full 7 ml EDTA sample Pediatric: 1 full 3 ml EDTA sample; neonate: 2 full 0.5 ml EDTA microtainers |
| Shipping Information | Ship at ambient temperature
Send samples to: |
| Requisition Form | Request for Testing-Immunohematology Reference Laboratory |
| Transaction Code | 3125-01 DAT monospecific IRL 3125-02 DAT polyspecific IRL |
| CPT Codes | 86880 |
| Test Schedule | Monday through Sunday |
| Turn around Time (analytic time) | 24 hours if weekday; 48 hours if weekend |
| Report/ Results | Fax number required to receive report |
